Although her ancestral family home is Taicang (in Jiangsu Province, China), she was born in 1912, in Shanghai, but was raised in Liuhe, a city about 30 miles from Shanghai. Her father, Wu Zhongyi (吳仲裔), was a proponent of gender equality and founded Mingde Women's Vocational Continuing School. She left her hometown at the age of eleven to go to the Suzhou Women's Normal School No. 2. Her mother was Fan Fuhua (樊復華).
She was admitted to the National Central University in Nanjing in 1929. According to the government regulations of the time, normal school students entering universities needed to serve as teachers for one year, so in 1929 she went to teach in the Public School of China (中國公學) founded by Hu Shi in Shanghai. From 1930 to 1934, she studied in the Physics Department of National Central University (renamed Nanjing University in 1949). For two years after her graduation, she did postgraduate study and worked as an assistant at Zhejiang University
At Columbia she contributed to the Manhattan Project by developing a process to separate uranium isotopes by gaseous diffusion and by developing improved Geiger counters. She assisted Tsung-Dao Lee personally in his parity laws development (with Chen Ning Yang) by providing him with a possible test method for beta decay in 1956 that worked successfully. Some consider this very instrumental in the creation of the laws, but she did not share their Nobel Prize – a fact widely blamed on sexism by the selection committee.
Her book Beta Decay (1965) is still a standard reference for nuclear physicists.
She later conducted research into the molecular changes in the deformation of hemoglobins that cause sickle-cell disease.
Wu set precedents for womankind on several occasions. She was: